SORT shRNA Vector Systems


品名: SORT shRNA Vector Systems

型號: shRNA

廠牌: Biosettia




Biosettia’s unique vector systems for gene silencing provide a time and cost saving method for delivering shRNA. Unlike other expression systems, which require two distinct sense/antisense shRNA oligos, only one palindromic oligo is required for annealing. The novelty of our pRNAi and pLV-RNAi vector systems offer users with several advantages over our competition。




* Our pLV-RNAi vectors are ready to use
No restriction digestion or vector purification is required. Additionally, our pLV-RNAi vectors are self-inactivated (SIN) vectors, which carry a U3 deletion in its 3′ LTR, eliminating its promoter activity. This self-inactivating deletion provides an additional level of safety by preventing the integrated viral genome from generating full-length lentiviral RNA.


* Reduced Cloning Complexity
Our single-strand DNA oligo encoding shRNA sequence is a perfect palindrome, thus (two) palindromic oligos can anneal to each other to form a double-stranded oligo. This eliminates the need to mix and anneal two different DNA oligos and reduces operational mistakes during the cloning process. The overall chance of mutations introduced during DNA oligo synthesis is reduced by 50%


* Low Cost
Only one DNA oligo is required to generate an shRNA expression clone. This setup is the most economical, and is distinguishable from all the other shRNA vectors commonly used in academia and industry, for which two different oligos are needed to make an shRNA expression clone. Also, the length of the DNA oligo used for pLV-RNAi (52-56 nt) is shorter than the length of the DNA oligos required for most of the other commercially available shRNA vectors.


* Highly Efficient, Low Background
The 5’-AAAA overhangs on annealed double-strand oligo can only be ligated to the 5’-TTTT overhangs on both ends of the linear pLV-RNAi vector. Unlike the overhangs generated by restriction digestion in other available shRNA expression vectors, these 5’-TTTT overhangs in pLV-RNAi will not self-ligate. As a result, the ligation of the double-strand oligo into the pLV-RNAi vector is highly efficient, and the contamination of empty, self-ligated pLV-RNAi vectors is greatly reduced.